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A Phase 1B Clinical Trial of Trametinib Plus Digoxin in Patients With Unresectable or Metastatic BRAF Wild-type Melanoma

NCT02138292

Description:

The study is a prospective, single-arm, one-site therapeutic trial of the combination of trametinib plus digoxin for advanced melanoma. endpoints are toxicities assessed by nCi CTCae v4.1 within the first 8 weeks, responses measured by ReCiST v1.1 criteria every 8 weeks with scans and exams, tumor sensitivity to the drug combination quantified by tumor regressions in nSG mice, and correlations of response with tumor sensitivity, BRaF status, MaPK inhibitor exposure history, and tumor sodium pump expression. Treatment Dosage and administration Study Drugs: 1. Trametinib (2mg) will be administered orally on a daily basis. 2. Digoxin (0.25mg) will be administered orally on a daily basis. on a 8-week cycle, duration of treatment can last from 8 to 104 weeks. endpoints 1. Toxicities will be assessed via nCi's CTCae v4.1 toxicity criteria. DLTs will be defined based on the rate of drug-related (definitely or probably) grade 3-5 adverse events experienced within the first 8 weeks of study treatment. The MTD will be exceeded if more than 20% of patients on the study experience DLTs. 2. Responses will be measured by ReCiST v1.1 every 8 weeks. Response duration will be defined as time from first documented response until disease progression. PFS is time from treatment until disease progression. 3. Patient tumor sensitivity to the drug combination will be quantified by the amount of subcutaneous established tumor growth inhibition in nSG mice by 5d/week oral gavage with drugs. 4. Tumor nRaS status will be determined by tumor Dna extraction, PCR amplification of exons and Sanger sequencing of nRaS. 5. History of prior MaPK inhibitor therapies will document MeK inhibitor exposures. 6. Sodium pump subunit expression will be analyzed by pretreatment tumor immunohistochemistry and a qualitative 0 to 3+ grading system.

Related Conditions:
  • Melanoma
Recruiting Status:

Completed

Phase:

Phase 1

Trial Eligibility

Document

A <span class="go-doc-concept go-doc-intervention">Phase 1B</span> Clinical Trial of <span class="go-doc-concept go-doc-intervention">Trametinib</span> Plus <span class="go-doc-concept go-doc-intervention">Digoxin</span> in Patients With Unresectable or Metastatic <span class="go-doc-concept go-doc-biomarker">BRAF</span> Wild-type <span class="go-doc-concept go-doc-disease">Melanoma</span>

Title

  • Brief Title: A Phase 1B Clinical Trial of Trametinib Plus Digoxin in Patients With Unresectable or Metastatic BRAF Wild-type Melanoma
  • Official Title: A Phase 1B Clinical Trial of Trametinib Plus Digoxin in Patients With Unresectable or Metastatic BRAF Wild-type Melanoma
  • Clinical Trial IDs

    NCT ID: NCT02138292

    ORG ID: 012014-007

    Trial Conditions

    Melanoma

    Trial Interventions

    Drug Synonyms Arms
    Trametinib (2mg)/Digoxin (.25mg) Trametinib (2mg)/Digoxin (0.25mg)

    Trial Purpose

    The study is a prospective, single-arm, one-site therapeutic trial of the combination of
    trametinib plus digoxin for advanced melanoma. endpoints are toxicities assessed by nCi
    CTCae v4.1 within the first 8 weeks, responses measured by ReCiST v1.1 criteria every 8
    weeks with scans and exams, tumor sensitivity to the drug combination quantified by tumor
    regressions in nSG mice, and correlations of response with tumor sensitivity, BRaF status,
    MaPK inhibitor exposure history, and tumor sodium pump expression.

    Treatment Dosage and administration

    Study Drugs:

    1. Trametinib (2mg) will be administered orally on a daily basis.

    2. Digoxin (0.25mg) will be administered orally on a daily basis.

    on a 8-week cycle, duration of treatment can last from 8 to 104 weeks.

    endpoints

    1. Toxicities will be assessed via nCi's CTCae v4.1 toxicity criteria. DLTs will be
    defined based on the rate of drug-related (definitely or probably) grade 3-5 adverse
    events experienced within the first 8 weeks of study treatment. The MTD will be
    exceeded if more than 20% of patients on the study experience DLTs.

    2. Responses will be measured by ReCiST v1.1 every 8 weeks. Response duration will be
    defined as time from first documented response until disease progression. PFS is time
    from treatment until disease progression.

    3. Patient tumor sensitivity to the drug combination will be quantified by the amount of
    subcutaneous established tumor growth inhibition in nSG mice by 5d/week oral gavage
    with drugs.

    4. Tumor nRaS status will be determined by tumor Dna extraction, PCR amplification of
    exons and Sanger sequencing of nRaS.

    5. History of prior MaPK inhibitor therapies will document MeK inhibitor exposures.

    6. Sodium pump subunit expression will be analyzed by pretreatment tumor
    immunohistochemistry and a qualitative 0 to 3+ grading system.

    Detailed Description

    Primary Objectives:

    - To describe the toxicities and estimate the frequency of dose limiting toxicities
    (DLTs) of digoxin in combination with trametinib in advanced melanoma patients and
    estimate the frequency of DLTs.

    - To measure the response rate, response duration and progression free survival (PFS) of
    digoxin plus trametinib in advanced melanoma.

    Secondary Objectives:

    - To correlate NSG xenograft sensivity to the drug combination with clinical response in
    the same patient.

    - To compare response rates in MAPK inhibitor naive versus refractory patients and NRAS
    mutant versus wild-type patients and for sodium pump 3 subunit high tumor expression
    versus low tumor expression patients.

    Rationale: Having established cell culture and xenograft systems for studying patient
    melanoma samples, the researchers were able to grow tumors in vitro and in vivo from single
    cells and found a correlation of tumor metastatic behavior in immunocompromised mice and in
    patients. Recently they have extended the experiments to examine melanoma sensitivity to
    novel compounds. In screens of FDA approved drugs, they found several cardenolides including
    digoxin that reproducibly exhibited greater toxicity to primary human melanomas as compared
    to a range of normal human cells. They then examined the anti-tumor efficacy against primary
    human melanomas growing in vivo as xenografts. While trametinib, vemurafenib, and digoxin
    and digitoxin individually slowed the growth of human melanomas xenografts, they did not
    cause tumor regression. However, the combination of digoxin or digitoxin and trametinib
    caused substantial tumor regression using melanomas obtained from multiple patients, some
    with BRAF mutations and some without. The effects were dramatically better than trametinib,
    digoxin, digitoxin or vemurafenib alone. No difference in efficacy for the combination was
    seen in BRAF mutant and BRAF wild-type samples.

    Trial Arms

    Name Type Description Interventions
    Trametinib (2mg)/Digoxin (0.25mg) Experimental Trametinib (2mg) will be administered orally on a daily basis. Digoxin (0.25mg) will be administered orally on a daily basis. On a 8-week cycle, duration of treatment can last from 8 to 104 weeks. Trametinib (2mg)/Digoxin (.25mg)

    Eligibility Criteria

    Inclusion Criteria:

    - 1. Histologic diagnosis of unresectable or metastatic melanoma. For unknown primary
    disease, diagnosis of metastatic disease by cytology FNA is not acceptable. BRAF
    wild-type confirmed, and NRAS mutation assessed.

    2. Age > 18 years.

    3. Any number of prior systemic therapeutic regimens for unresectable stage III or
    stage IV melanoma. This includes chemotherapy, immunotherapy, pathway inhibitors,
    biochemotherapy, or investigational treatments. Patients may also have received
    therapies in the adjuvant setting.

    4. ECOG Performance status 0-2.

    5. Adequate organ and marrow function as defined below:

    - leukocytes 2,000/mcL

    - absolute neutrophil count 1,000/mcL

    - platelets 75,000/mcl

    - total bilirubin < 3 x institutional upper limit of normal

    - AST(SGOT)/ALT(SPGT) 2.5 X institutional upper limit of normal

    - creatinine < 1.5 mg/dL

    - cardiac ejection fraction > 50%

    - QTc < 480msec

    6. Women of child-bearing potential and men must agree to use adequate contraception
    (hormonal or barrier method of birth control; abstinence) prior to study entry, for
    the duration of study participation, and for 90 days following completion of therapy.
    Should a woman become pregnant or suspect she is pregnant while participating in this
    study, she should inform her treating physician immediately. A female of
    child-bearing potential is any woman (regardless of sexual orientation, having
    undergone a tubal ligation, or remaining celibate by choice) who meets the following
    criteria: Has not undergone a hysterectomy or bilateral oophorectomy; or Has not been
    naturally postmenopausal for at least 12 consecutive months (i.e., has had menses at
    any time in the preceding 12 consecutive months).

    7. All sites of disease must be evaluated within 4 weeks prior to beginning therapy.
    Patients must have measurable disease as defined by RECIST v1.1.

    8. Ability to understand and the willingness to sign a written informed consent.

    9. Patients must be willing to undergo tumor biopsy pretreatment and at relapse.

    Exclusion Criteria:

    1. Subjects who have had chemotherapy or radiotherapy or any systemic therapy for
    melanoma within 2 weeks prior to entering the study or those who have not recovered
    from adverse events due to agents administered more than 2 weeks earlier. No
    concomitant therapy is allowed including IL2, interferon, ipilimumab, anti-PD-1 or
    anti-PD-L1 antibody, cytotoxic chemotherapy, immunosuppressive agents, or other
    investigational therapies.

    2. Active infection with hepatitis B or C or HIV.

    3. Subjects with active CNS disease are excluded. Patient with brain metastases
    previously treated with surgery or radiation therapy and with confirmed SD for >4
    weeks are allowed.

    4. Patients are excluded if they have a history of any other malignancy from which the
    patient has been disease-free for less than 2 years, with the exception of adequately
    treated and cured basal or squamous cell skin cancer, superficial bladder cancer or
    carcinoma in situ of the cervix.

    5. Uncontrolled inter-current illness including, but not limited to, ongoing or active
    infection, symptomatic congestive heart failure, unstable angina pectoris, cardiac
    arrhythmia, or psychiatric illness/social situations that would limit compliance with
    study requirements. Cardiac symptoms or events within 24 weeks.

    6. History of predisposition to retinal vein occlusion or central serous retinopathy.

    7. Inability to assess BRAF or NRAS mutation status. Hypersensitivity to digoxin.

    8. Wolff-Parkinson White syndrome or AV block or sinus node dysfunction.

    Minimum Eligible Age: 19 Years

    Maximum Eligible Age: N/A

    Eligible Gender: Both

    Primary Outcome Measures

    Dose Limiting Doxicities (DLTs)

    Secondary Outcome Measures

    Response Rate

    Trial Keywords