The technology now exists to advance the concept of personalized medicine for all cancer
patients. The advent of next generation sequencing can allow us to potentially characterize
each individual's tumor for oncogenic driver mutations. It is unknown at present if a wide
range of mutations in the phosphatidylinositol 3-kinase (PI3K) pathway predict for
responsiveness to a PI3K inhibitor as would be expected in a situation of "oncogene
addiction". In this study, we plan to explore two cohorts of patients and response to BYL719,
an oral selective PI3K-alpha inhibitor: 1. Metastatic triple negative breast cancer (triple
negative breast cancer [TNBC] which is defined as ER-/HER2-) and 2. PI3K pathway abnormal
metastatic luminal breast cancer (ER+/HER2-). Both cohorts are eligible for this study after
progression after standard first line therapy. In recent large sequencing efforts, for
example The Cancer Genome Atlas [TCGA], primary breast cancers were found to have a large
number of Pi3K pathway abnormalities- in TNBC there was a high rate of genetic abnormalities
in the Pi3K pathway (combined mutations, amplifications and deletions in nearly 100% of
TNBC)- as well as in ER+/HER2- disease. Therefore, in this study we plan to study responses
to BYL719 and associations with genetic features that could signify Pi3K pathway activation
in a cohort of fully molecular- characterized metastatic breast cancers in order to identify
biomarkers of response to selective PI3K alpha inhibition in breast cancer.
- Patients eligible for inclusion in this study have to meet all of the following
- Males and females of any menopausal status
- Patient has signed the Informed Consent Form (ICF) prior to any screening procedures
being performed and is able to comply with protocol requirements
- Age ≥ 18 years old
- Eastern Cooperative Oncology Group (ECOG) 0-2 that the investigator believes is stable
at the time of screening
- Patient has locally recurrent (incurable) or metastatic disease
- Patient is able to swallow and retain oral medication
- Known HER2 status (local lab) that is negative on IHC (IHC=0) and/or non-amplified.
- Known estrogen receptor (ER) and progesterone receptor (PR) status (local lab)
- Recent tumor tissue must be available from a metastatic or recurrent lesion for next
generation sequencing targeted gene panel
- Patients with TNBC disease (ER<1%, HER2-negative) should have documented progression
on at least one line of prior systemic therapy in the metastatic setting or within 12
months of adjuvant therapy completion. There is no limit on previous therapies. There
will be no molecular selection of these patients.
- Patients with ER-positive (ER≥1%, HER2-negative) disease should have documented
progression on at least one line of prior systemic endocrine therapy in the metastatic
setting. There is no limit on previous therapies. Prior everolimus is allowed.
- Patients are defined as "PI3K abnormal" if they have documented gene mutation in
AKT1,2,3,ALK, EGFR, ERBB2,3,4, HRAS, INPP4B, KRAS, NRAS, PTEN, PIK3CA, PIK3R1, PIK3R3,
PTEN or gene amplification in EGFR, PIK3CA, PIK3R1 or loss in PTEN and INPP4B as per a
next generation targeted gene sequencing panel
- Measurable disease by RECIST v 1.1 criteria or non- measurable disease that is
clinically evaluable (bone only disease allowed if evaluable)
- Patient has adequate bone marrow and organ function assessed within 72 hours prior to
- Absolute neutrophil count (ANC) ≥ 1.5 x 10^9/L
- Platelets ≥ 100 x 10^9/L
- Hemoglobin (Hgb) ≥ 9.0 g/dL
- Serum creatinine ≤ 1.5 x ULN
- Total serum bilirubin ≤ 1.5 x ULN (in patients with known Gilbert's syndrome, a total
bilirubin ≤ 3.0 x ULN with direct bilirubin ≤ 1.5 x ULN)
- AST and ALT ≤ 2.5 x ULN (alternatively < 5 x ULN if evidence of liver metastases)
- Fasting blood glucose ≤ 140mg/dL or ≤ 7.8 mmol/L
Patients eligible for this study must not meet any of the following criteria:
- Patient has a primary CNS tumor or CNS tumor involvement.
- However patients with metastatic CNS tumors may participate in this study if the
- Four weeks from prior therapy completion (including radiation and surgery) to starting
- Clinically stable with respect to the CNS tumor at the time of screening
- Not receiving steroid therapy
- Patient with diabetes mellitus (fasting glucose >120mg/dl or 6.7 mmol/L), or
documented steroid-induced diabetes mellitus
- Patient has a history of another malignancy within 2 years prior to starting study
treatment, except for cured basal cell carcinoma of the skin or excised carcinoma in
situ of the cervix.
- Patient who has not recovered to grade 1 or better (except alopecia) from related side
effects of any prior antineoplastic therapy
- Patient who has had systemic chemotherapy within 4 weeks (6 weeks for nitrosoureas or
mitomycin C) prior to study entry.
- Patient who has received radiotherapy ≤ 4 weeks prior to starting study drugs, with
exception of palliative radiotherapy (≤ 2 weeks prior to starting study drugs), who
has not recovered from side effects of such therapy to baseline or Grade ≤ 1 and/or
from whom ≥ 30% of the bone marrow was irradiated. Target lesions should not have had
previous irradiation unless have progressed post treatment.
- Patient who has undergone major surgery ≤ 4 weeks prior to starting study treatment or
who has not recovered from side effects of such procedure.
- Patient has a clinically significant cardiac disease or impaired cardiac function,
- Congestive heart failure (CHF) requiring treatment (New York Heart Association (NYHA)
Grade ≥ 2), left ventricular ejection fraction (LVEF) < 50% as determined by
multi-gated acquisition (MUGA) scan or echocardiogram (ECHO)
- History or current evidence of clinically significant cardiac arrhythmias, atrial
fibrillation and/or conduction abnormality, e.g. congenital long QT syndrome,
- Acute coronary syndromes (including myocardial infarction, unstable angina, coronary
artery bypass graft (CABG), coronary angioplasty, or stenting), < 3 months prior to
- QT interval adjusted according to Fredericia (QTcF) > 480 msec on screening ECG.
- Patient who has any severe and/or uncontrolled medical conditions such as:
- Active or uncontrolled severe infection,
- Liver disease such as cirrhosis, decompensated liver disease, and chronic hepatitis
(i.e. quantifiable HBV-DNA and/or positive HbsAg, quantifiable HCV-RNA)
- Known severely impaired lung function (spirometry and DLCO 50% or less of normal and
O2 saturation 88% or less at rest on room air)
- Active, bleeding diathesis;
- Uncontrolled arterial hypertension defined by blood pressure > 140/100 mm Hg at rest
(average of 3 consecutive readings 5 min apart)
- Chronic treatment with corticosteroids or other immunosuppressive agent
- Patient who is currently receiving medication with a known risk of prolonging the QT
interval or inducing Torsades de Pointes (TdP) and the treatment cannot either be
discontinued or switched to a different medication prior to starting study drug
- Patient who has participated in a prior investigational study within 30 days prior to
- Patient who is currently receiving treatment with drugs known to be moderate or strong
inhibitors or inducers of isoenzymes CYP34A or CYP2C8. The patient must have
discontinued moderate and strong inducers of both enzymes for at least one week and
must have discontinued strong and moderate inhibitors before the start of treatment.
Switching to a different medication prior to start of treatment is allowed; Refer to
- Patient with impaired gastrointestinal (GI) function or GI disease that may
significantly alter the absorption of oral BYL719 (e.g. ulcerative disease,
uncontrolled nausea, vomiting, diarrhea, malabsorption syndrome, or small bowel
- Patient with known positive serology for human immunodeficiency virus (HIV).
- Patients who have received live attenuated vaccines within 1 week of start of study
drug and during the study. Patient should also avoid close contact with others who
have received live attenuated vaccines. Examples of live attenuated vaccines include
intranasal influenza, measles, mumps, rubella, oral polio, BCG, yellow fever,
varicella and TY21a typhoid vaccines.
- Pregnant or nursing (lactating) woman, where pregnancy is defined as the state of a
female after conception and until the termination of gestation, confirmed by a
positive hCG laboratory test (> 5 mIU/mL).
- Patient who does not apply highly effective contraception during the study and through
the duration as defined below after the final dose of study treatment.