The purpose of this study is to determine the safety and efficacy of Tumor Associated Peptide
Antigen (TAPA) pulsed dendritic cell (DC) vaccines in the treatment of hematologic
malignancies.
Patients diagnosed with hematologic malignancies (as defined) who have responded to
conventional therapy, and without any potentially curative therapeutic intervention, will be
candidates for this Phase I/II study. Following confirmation of disease response to
conventional antineoplastic therapy, eligible patients who agree to participate and sign a
consent form will have their tumor cells and/or blood analyzed for the expression of a
specific panel of Tumor Associated Peptide Antigens (TAPAs), including SP17, Ropporin, AKAP4,
PTTG1 and Span-xb. Patients whose tumors express one (1) or more of these TAPAs will receive
three (3) days of subcutaneous Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) to
increase bone marrow production of monocytes and dendritic cell (DC) precursors, and
peripheral blood mononuclear cells will be obtained by phlebotomy and/or leukapheresis for
generation of autologous DCs. Patient's DCs will be generated at Kiromic's Cell Processing
Good Manufacturing Process (GMP) facility, according to established Standard Operating
Procedures, and activated by pulsing/loading them with the TAPA(s) relevant for each
particular patient. Patients will receive five (5) days of low-dose cyclophosphamide prior to
each vaccination with TAPA-pulsed DCs to decrease Treg activity. TAPA-pulsed DCs will be
administered at a fixed dose of up to 1 x 107 DCs at least two (2) days following
cyclophosphamide administration. DC vaccination schedule will be once every fourteen (14)
days via subcutaneous (SC) and intradermal (ID) injections for a total of 6 vaccinations. Low
dose GM-CSF will also be administered SC for five (5) consecutive days, starting three (3) to
six (6) hours after each TAPA-pulsed DC treatment, to optimize immune response and DC
viability in vivo. Patients will be followed on a weekly basis (or more frequently if
required) to evaluate treatment-related toxicity. Immune responses and anti-tumor responses
will be evaluated per protocol specifications. Continuation and stopping rules for the study
will be defined based on toxicity/tolerability (Phase I) and/or immune responses (Phase II).
Inclusion Criteria:
1. Ability to provide informed consent.
2. Patients at least eighteen (18) years of age with histologically or cytologically
proven Multiple Myeloma (MM), Hodgkins Disease (HD), Non-Hodgkins Lymphoma (NHL),
Chronic Myelogenous Leukemia (CML), Acute Lymphocytic Leukemia (ALL), Acute
Myelogenous Leukemia (AML) and Chronic Lymphocytic Leukemia (CLL), who have responded
to standard, first-line antineoplastic therapy, as defined using standard response
criteria for the specific hematologic malignancy (HM), and have no additional
potentially curative therapeutic intervention available, will be eligible to
participate in this study.
3. Expression of one (1) or more of the following TAPAs: SP17, AKAP4, Ropporin, PTTG1 and
Span-xb, by either reverse transcriptase polymerase chain reaction (RT-PCR) and/or
immunocytochemistry, Western blotting or ELISA, in neoplastic cells and/or blood.
4. Presence of measurable or evaluable disease (unless patient has achieved a complete
response (CR) following first-line antineoplastic therapy).
5. Patients must not have any active infectious process.
6. Patients must have a negative test for HIV, Hepatitis A, B, and C.
7. Patients must not be receiving active immunosuppressive therapy.
8. Patients must have discontinued systemic antineoplastic therapy (including systemic
corticosteroids and excluding tyrosine kinase inhibitors for CML) at least four (4)
weeks prior to enrollment.
9. Patients may not have any known allergy to CYP and/or GM-CSF.
10. Patients must be willing to provide at least 250 mL, and up to 500 mL, of whole blood
obtained by phlebotomy and/or consent to leukapheresis for DC generation.
11. Adequate renal and hepatic function (creatinine ≤ 2.0 mg/dl, bilirubin ≤ 2.0 mg/dl,
aspartate transaminase (AST) and alanine transaminase (ALT) ≤ 4X upper limit of normal
range).
12. Adequate hematologic function (Platelets ≥ 60,000/mm3, lymphocytes ≥ 1,000/mm3,
neutrophils ≥ 750/mm3, hemoglobin ≥ 10 g/dl).
13. Karnofsky performance status ≥ 70%.
14. Expected survival ≥ 6 months.
15. Patient Human Leukocyte Antigen (HLA) typing should demonstrate HLA-A*01, and/or
HLA-A*02, and/or HLA-A*24 restriction.
16. Either a female or male of reproductive capacity wishing to participate in this study
must be using, or agree to use, one or more types of birth control during the entire
study and for 3 months after completing the study. Birth control methods may include
condoms, diaphragms, birth control pills, spermicidal gels or foams, anti-gonadotropin
injections, intrauterine devices (IUD), surgical sterilization, or subcutaneous
implants. Another choice is for a subject's sexual partner to use one of these birth
control methods. Women of reproductive capacity will be required to undergo a urine
pregnancy test before completion of the post-screening informed consent process.
Exclusion Criteria:
1. Patients with HM, as previously defined, without confirmed response to standard,
first-line antineoplastic therapy, and/or who do not fulfill all Inclusion Criteria as
stated, will be ineligible to participate in this study.
2. Patients with HM who have undergone myeloablative systemic therapy are ineligible to
participate in this study.
3. Patients without measurable or evaluable disease (unless patients achieved a complete
response (CR) following 1st-line antineoplastic therapy).
4. Patients receiving cytotoxic therapy, radiation therapy, immunotherapy or non-topical
steroids for HM within four (4) weeks of enrollment, excluding tyrosine kinase
inhibitors in patients with CML.
5. Active immunosuppressive or cytotoxic therapy (excluding topical steroids) for any
other condition.
6. Persistent fever (>24 hours) documented by repeated measurement or active,
uncontrolled infection within 4 weeks of enrollment.
7. Active ischemic heart disease or history of myocardial infarction within six months.
8. Active autoimmune disease, including, but not limited to, Systemic Lupus Erythematosus
(SLE), Multiple Sclerosis (MS), Ankylosing Spondylitis (AS), and Rheumatoid Arthritis
(RA).
9. Pregnancy or breast feeding.
10. Patients with an active second invasive malignancy, other than basal cell carcinoma of
the skin.
11. Life expectancy of less than 6 months.
12. Patients with contraindications to CYP and/or GM-CSF.
13. Patients who have received organ transplantations.
14. Patients with psychological or geographic conditions that prevent adequate follow-up
or compliance with the study protocol.
15. Patients diagnosed with primary central nervous system (CNS) or with CNS
metastases/involvement, at any time during the disease course, are excluded from the
study.
16. Patients with HLA-A alleles not belonging to any of the following subtypes: HLA-A*01,
or HLA-A*02, or HLA-A*24.
