The purpose of this research study is to determine the acceptable upper limit dose of
nivolumab in combination with dasatinib that may be given to patients with
relapsed/refractory philadelphia chromosome positive acute lymphoblastic leukemia (Ph+ ALL).
Nivolumab is currently Food and Drug Administration (FDA) approved for other cancers, but has
not yet been investigated in Ph+ ALL. Dasatinib is currently FDA approved for the treatment
of Ph+ ALL, but has not yet been investigated in combination with nivolumab for this disease.
There is evidence that dasatinib not only blocks the Philadelphia chromosome or breakpoint
cluster region-Abelson murine leukemia viral oncogene homolog 1 (BCR-ABL) mutation, but also
increases the activity of cells in your immune system. Nivolumab increases T cells in your
immune system, which allows your immune system to attack the cancer. We think the combination
of these drugs will be more effective against your leukemia than either drug used alone.
PRIMARY OBJECTIVES:
I. To determine the maximum tolerated dose (MTD) of nivolumab when given in combination with
dasatinib in patients with relapsed/refractory Philadelphia chromosome positive (Ph+) acute
lymphoblastic leukemia (ALL).
SECONDARY OBJECTIVES:
I. To evaluate the toxicities and safety profile of nivolumab and dasatinib in patients with
relapsed/refractory Ph+ ALL.
II. To determine the rate of complete hematologic remission (CR) after three cycles of
nivolumab and dasatinib.
III. To determine the rate of molecular remission after three cycles of nivolumab and
dasatinib.
IV. To study the pharmacokinetics of nivolumab and dasatinib. V. To evaluate programmed cell
death 1 (PD1) expression levels and saturation in the peripheral blood and bone marrow.
VI. To measure peripheral T-cell levels and activation in response to treatment.
TERTIARY OBJECTIVES:
I. To evaluate the 30 day mortality rate, overall survival (OS), progression free survival
(PFS), and duration of remission (DOR) one year after treatment with nivolumab when given in
combination with dasatinib in patients with relapsed/refractory Ph+ ALL.
II. To compare the OS between patients who receive a hematopoietic stem cell transplant and
those who receive no further therapy following remission.
III. To evaluate for resistance mutations at the time of disease progression.
OUTLINE:
Patients receive dasatinib orally (PO) once daily (QD ) on days 1-28 and nivolumab
intravenously (IV) over 30 minutes on days 8 and 22 of course 1 and on days 1 and 15 of
subsequent courses. Courses repeat every 28 days in the absence of disease progression,
unacceptable toxicity, or withdrawal from the study for other reasons.
After completion of study treatment, patients are followed up at 30 days and then monthly for
up to 1 year.
Inclusion Criteria:
- Patients must have a histologically confirmed diagnosis of Ph+ ALL
- Detection of one of the following must be present:
- t(9;22)(q34;q11) or 3-way variant by metaphase cytogenetics
- Breakpoint cluster region (BCR)-Abelson (ABL) positive status by molecular
analysis with qualitative polymerase chain reaction (PCR) or fluorescence in situ
hybridization (FISH)
- Patients must have primary refractory ALL based on failure to achieve a hematologic or
molecular remission after induction therapy with dasatinib and steroids or dasatinib
and chemotherapy, or have relapsed after treatment with a tyrosine kinase inhibitor
with or without chemotherapy
- Note: Prior course of dasatinib and steroid induction therapy should have
included dasatinib 140mg PO daily on days 1-84 and prednisone 60mg/m^2 (capped at
120mg, or equivalent steroid dose) on days 1-28; if patients were unable to
tolerate full steroid dose during induction therapy they will still be eligible
- Note: Patients with refractory or relapsed disease in the central nervous system
will be eligible
- Prior chemotherapy or tyrosine kinase inhibitor (TKI) treatment, aside from dasatinib,
must be >= 7 days before first investigational agent dose
- Patients must exhibit an Eastern Cooperative Oncology Group (ECOG) performance status
of 0-2
- Patients must have adequate organ and bone marrow function prior to registration, as
defined below:
- Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) < 2 x
institutional upper limit of normal (IULN)
- Total bilirubin < 2.0 x IULN (unless Gilbert syndrome has been diagnosed); if
leukemia infiltration of the liver is suspected to be causing liver function
abnormalities the patient will still be eligible with principal investigator (PI)
approval
- Creatinine < 2 x IULN
- Creatinine clearance > 40 mL/min (measured by Cockroft-Gault)
- Females of child-bearing potential (FOCBP) must have a negative pregnancy test within
7 days of registration
- Note: A FOCBP is any woman (regardless of sexual orientation, having undergone a
tubal ligation, or remaining celibate by choice) who meets the following
criteria:
- Has not undergone a hysterectomy or bilateral oophorectomy
- Has had menses at any time in the preceding 12 consecutive months (and
therefore has not been naturally postmenopausal for > 12 months)
- Women must not be breastfeeding at the time of study registration
- Women and men of reproductive potential should agree to use two effective means of
birth control
- For women, contraception should continue for 23 weeks after the last dose of
nivolumab and 12 weeks after the last dose of dasatinib to allow complete
clearance of drug and its principal metabolites from the body
- For men, contraception should continue for 31 weeks after nivolumab and 12 weeks
after dasatinib
- Patients must have the ability to understand and the willingness to sign a written
informed consent prior to registration on study
Exclusion Criteria:
- Patients may not be receiving any other investigational agents within 5 half-lives of
the drug (if known); if the half-life is not known, investigational agents should not
be taken within two weeks
- Patients are not eligible if they have an intolerance to most recent prior TKI (other
than dasatinib) at the lowest possible effective dose, defined as a grade >= 3
toxicity considered at least possibly related to that TKI; patients are also excluded
if they are intolerant or allergic to dasatinib and discontinued prior therapy due to
a >= grade 2 treatment related adverse event
- Patients must not have a history of a grade 4 anaphylactic reaction to monoclonal
antibody therapy or known hypersensitivity reactions to drugs formulated with
polysorbate 90
- Patients must not have had any prior therapy with an anti-PD-1, anti-programmed cell
death 1 ligand 1 (PD-L1), anti-programmed cell death 1 ligand 2 (PD-L2), anti-cluster
of differentiation (CD)137 or anti-cytotoxic t-lymphocyte-associated protein 4 ligand
(CTLA-4) antibody (or any antibody or drug specifically targeting T-cell costimulation
or checkpoint pathways; for questions or uncertainties, please contact the PI or
quality assurance manager (QAM)
- Patients who have had allogeneic hematopoietic stem cell transplant (HSCT) are not
eligible if they meet any of the following:
- transplant is within 2 months from cycle 1, day 1 (C1D1)
- Has clinically significant graft-versus-host disease requiring treatment
- Has >= grade 3 persistent non-hematological toxicity related to the transplant
- Concomitant use of strong inhibitors of the cytochrome p450, family 3, subfamily a,
polypeptide 4 (CYP3A4) isoenzyme is not permitted; must have wash-out period of 5
times the half-life of the compound before first dasatinib dose
- Concomitant use of QT prolonging agents strongly associated with torsades de pointes
is not permitted
- Patients who have a known dasatinib-resistant ABL-kinase mutation such as T315I are
not eligible; for confirmation, please contact PI
- Patients who have any serious or uncontrolled medical disorder that would impair the
ability of the subject to receive protocol therapy are not eligible; these include,
but are not limited to:
- Active infection that is not well controlled
- Known pleural or pericardial effusion at baseline
- Clinically significant gastrointestinal disease or digestive dysfunction
compromising absorption of dasatinib
- Pulmonary arterial hypertension
- Uncontrolled or significant cardiovascular disease, including:
- Myocardial infarction within 6 months of enrollment date
- Uncontrolled angina or congestive heart failure within 3 months of
enrollment date
- Left ventricular ejection fraction (LVEF) < 40%
- Significant cardiac conduction abnormality, including:
- History of clinically significant ventricular arrhythmia (such as
ventricular tachycardia, ventricular fibrillation, or torsades de
pointes)
- History of second or third degree heart block (except for second degree
type 1)
- Corrected QT (QTc) interval > 500 msec, unless a cardiac pacemaker is
present
- Prior malignancy active within the previous 3 years, except for locally curable
cancers that have been apparently cured, such as basal or squamous cell skin
cancers, superficial bladder cancer, or carcinoma in situ of the prostate, cervix
or breast
- Subjects with active, known or suspected autoimmune disease; (Note: Subjects with
vitiligo, type I diabetes mellitus, hypothyroidism due to autoimmune condition
only requiring hormone replacement, psoriasis not requiring systemic treatment,
or conditions not expected to recur in the absence of an external trigger are
permitted to enroll)
- Psychiatric illness/social situations that would limit compliance with study
requirements
- Any other illness or condition that the treating investigator feels would
interfere with study compliance or would compromise the patient's safety or study
endpoints
- Female patients who are pregnant or nursing are not eligible
- Patients are not eligible if they have a known positive test for hepatitis B virus
surface antigen (HBV sAg) or hepatitis C virus ribonucleic acid (hepatitis C virus
[HCV] antibody) indicating acute infection; Note: Patients with evidence of chronic
hepatitis B infection will be allowed to enroll if on appropriate suppressive
medications under the direction of a hepatologist and with PI approval
- Patients who are known to be positive for human immunodeficiency virus (HIV) or known
acquired immunodeficiency syndrome (AIDS) are not eligible
- Patients must not have live vaccine therapies for prevention of infectious diseases
within 28 days of first nivolumab dose
- Patients who are unable to swallow oral medication are not eligible
- Patients with active autoimmune disease or history of autoimmune disease that might
recur, which may affect vital organ function or require immune suppressive treatment
including chronic prolonged systemic corticosteroids (defined as corticosteroid use of
duration one month or greater), should be excluded; these include but are not limited
to patients with a history of:
- Immune related neurologic disease
- Multiple sclerosis
- Autoimmune (demyelinating) neuropathy
- Guillain-Barre syndrome
- Myasthenia gravis
- Systemic autoimmune disease such as systemic lupus erythematosus (SLE)
- Connective tissue diseases
- Scleroderma
- Inflammatory bowel disease (IBD)
- Crohn's
- Ulcerative colitis
- Patients with a history of toxic epidermal necrolysis (TEN)
- Stevens-Johnson syndrome
- Anti-phospholipid syndrome NOTE: Subjects with vitiligo, type I diabetes
mellitus, residual hypothyroidism due to autoimmune condition only requiring
hormone replacement, psoriasis not requiring systemic treatment, or conditions
not expected to recur in the absence of an external trigger are permitted to
enroll
