Study RGX-104-001 is a Phase 1, first-in-human, dose escalation and expansion study of
RGX-104, an oral small molecule targeting the liver X receptor (LXR), as a single agent and
in combination with nivolumab, ipilimumab, docetaxel, or pembrolizumab plus
carboplatin/pemetrexed. RGX-104 activates LXR, resulting in depletion of both myeloid-derived
suppressor cells (MDSCs) as well as tumor blood vessels. MDSCs block the ability of T-cells
and other cells of the immune system from attacking tumors.
During the dose escalation stage, multiple doses and schedules of orally administered RGX-104
with or without nivolumab, ipilimumab, docetaxel, or pembrolizumab plus
carboplatin/pemetrexed (single agent or combination therapy) will be evaluated in patients
with advanced solid tumors and lymphoma (i.e., locally advanced and unresectable, or
metastatic) who have had progressive disease (PD) on available standard systemic therapies or
for which there are no standard systemic therapies of relevant impact. Dose escalation in
combination with pembrolizumab plus carboplatin/pemetrexed will be restricted to patients
with non-small cell lung cancer (NSCLC).
In the expansion stage of the study, additional patients with epithelial ovarian carcinoma
(EOC), NSCLC, or small cell lung cancer (SCLC)/high-grade neuroendocrine tumors (HG-NET) will
be treated at the MTD (or maximum tested dose if no MTD is identified, or dose below the MTD
if there is evidence suggesting a more favorable risk/benefit profile). This stage will
provide further characterization of the safety, efficacy, PK, and pharmacodynamics, including
biomarkers of immunologic activity and LXR target activation, of RGX-104 as a single agent
(EOC), in combination with docetaxel (SCLC/HG-NET), and and in combination with pembrolizumab
plus carboplatin/pemetrexed (NSCLC).
1. The patient must have histologic or cytologic evidence of a malignant solid tumor or
lymphoma (any histology) and must have advanced disease, defined as cancer that is
either metastatic or locally advanced and unresectable (and for which additional
radiation therapy or other locoregional therapies are not considered feasible).
2. With the exception of dose escalation with pembrolizumab plus carboplatin/pemetrexed,
patients enrolled in the dose escalation stages must have disease that is resistant to
or relapsed following available standard systemic therapy, or for which there is no
standard systemic therapy or reasonable therapy in the physician's judgment likely to
result in clinical benefit or if such therapy has been refused by the patient.
Documentation of the reason must be provided for patients who have not received a
standard therapy likely to result in clinical benefit.
3. Patients enrolled in the expansion stages must agree to a tumor biopsy to be obtained
during the screening period and toward the beginning of Cycle 2 or at the time of PD,
if earlier. If a biopsy is deemed by the investigator to not be in the patient's best
interest, prior approval must be obtained from the Medical Monitor to waive this
4. The patient must have disease that is measurable by standard imaging techniques per
RECIST or immune-related response criteria (irRC; all tumor types except lymphoma) or
International Working Group (IWG) revised response criteria for malignant lymphoma
(lymphoma only). For patients with prior radiation therapy, measurable lesions must be
outside of any prior radiation field(s), unless disease progression has been
documented at that disease site subsequent to radiation.
5. The patient is ≥18 years old.
6. The patient has an ECOG PS of ≤1.
7. The patient has adequate baseline organ function, as demonstrated by the following:
- Serum creatinine ≤1.5 × institutional upper limit of normal (ULN) or calculated
creatinine clearance >30 mL/min (>45 ml/min for patients receiving
- Serum albumin ≥2.5 g/dl;
- Bilirubin ≤1.5 × institutional ULN.
- Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) ≤2.5 ×
institutional ULN. Patients enrolled in an expansion stage may have ALT and AST <
5 × institutional ULN if the patient has hepatic metastases;
- For patients not taking warfarin or other oral anticoagulants: international
normalized ratio (INR) ≤1.5 or prothrombin time (PT) ≤1.5 × ULN; and either
partial thromboplastin time or activated partial thromboplastin time (PTT or
aPTT) ≤1.5 × ULN. Patients taking warfarin should be on a stable dose that
results in a stable INR <3.5. Among patients receiving other oral anticoagulant
therapy, PT or aPTT must be within the intended therapeutic range of the
8. The patient has adequate baseline hematologic function, as demonstrated by the
- Absolute neutrophil count (ANC) ≥1.5×109/L;
- Hemoglobin ≥8 g/dL and no red blood cell (RBC) transfusions during the prior 14
- Platelet count ≥100×109/L and no platelet transfusions during the prior 14 days.
9. The patient has a normal left ventricular ejection fraction (LVEF) per institutional
criteria as determined by either echocardiography (ECHO) or multigated acquisition
10. If the patient is a woman of child-bearing potential (WOCBP), she has had a negative
serum or urine pregnancy test within 2 weeks prior to treatment.
11. The patient (men and WOCBP) agrees to use acceptable contraceptive methods for the
duration of time on the study, and continue to use acceptable contraceptive methods
for 1 month after the last dose of study therapy. Patients receiving combination
therapy must agree to use acceptable contraceptive methods for the duration of time on
the study and continue to use acceptable contraceptive methods for 6 months after the
last dose of study therapy.
12. The patient has signed informed consent prior to initiation of any study-specific
procedures or treatment.
13. The patient is able to adhere to the study visit schedule and other protocol
requirements, including follow-up for survival assessment.
14. Tumor tissue (a minimum of 10 and up to 15 unstained slides, or paraffin block,
ideally from the patient's most recent biopsy, must be made available prior to the
first dose of study therapy.
15. For patients with EOC enrolled in the single agent expansion stage:
- The patient must have a pathologically confirmed (by histology or cytology)
diagnosis of epithelial ovarian, fallopian tube or primary peritoneal carcinoma,
which is currently recurrent or persistent Stage 3 or Stage 4 disease. A
histologic diagnosis of borderline, low malignant potential epithelial carcinoma
is not permitted.
- The patient must have received an appropriate platinum-based chemotherapy in the
first line setting.
- If the patient has platinum-sensitive relapsed disease (first relapse > 6 months
from end of initial platinum therapy), the patient should have been re-treated
with platinum for relapsed disease (or be intolerant or have refused such
- The patient may not have received treatment with immune checkpoint inhibitors
(e.g., products that target PD-L1, PD-1, or CTLA-4).
- The patient must not have required a paracentesis within the preceding 4 weeks
nor be projected to require a paracentesis within the next 8 weeks.
16. For patients with NSCLC enrolled in the pembrolizumab plus carboplatin/pemetrexed
combination dose escalation or expansion stages:
- The patient must have histologic or cytologic evidence of newly-diagnosed
non-squamous, NSCLC that is advanced disease, defined as cancer that is either
metastatic (Stage 4) or locally advanced (Stage 3B) and unresectable.
- The patient has confirmation that epidermal growth factor receptor (EGFR) or
anaplastic lymphoma kinase (ALK)-directed therapy is not indicated.
- The patient has not received prior systemic treatment for their
- For patients in the expansion stage only: the patient's tumor block must
demonstrate PD-L1 expression TPS <1% as determined with a validated assay.
- The patient must have adequate organ function and performance status eligible for
treatment with a platinum-based regimen and checkpoint inhibitor.
17. For patients with SCLC enrolled in the docetaxel combination expansion stage:
- The patient must have a pathologically confirmed (by histology or cytology)
diagnosis of SCLC, which is currently extensive disease (disease outside a single
radiation port), or of metastatic neuroendocrine cancer with small cell or
high-grade features. Patients with metastatic neuroendocrine cancer with small
cell or high-grade features must have a pathology report supplied to the sponsor
- The patient must have demonstrated disease progression following platinum-based
chemotherapy with or without a PD-1/L1 inhibitor for SCLC, or following a
different, acceptable first-line regimen for high-grade neuroendocrine tumors.
- The patient must have received no more than 1 prior line of therapy for extensive
1. The patient has persistent clinically significant toxicities (Grade ≥2) from previous
anticancer therapy (excluding Grade 2 chemotherapy-related neuropathy and alopecia
which are permitted). Prior toxicities that resulted in laboratory abnormalities
should have resolved to Grade ≤1, unless a higher-grade abnormality is allowed by the
inclusion criteria. If medical therapy is required for the treatment of a laboratory
abnormality, the dose and laboratory value(s) should be stable.
2. If considered for combination therapy with nivolumab or ipilimumab, the patient has:
- Uncontrolled clinically significant pulmonary disease.
- A history of any grade immune-related ocular event.
- A history of Grade ≥3 immune-related adverse event regardless of offending agent.
- Active autoimmune disease that required systemic treatment in the past. Patients
who have not required systemic treatment for at least two years may be enrolled
if permission is provided after discussion with the Medical Monitor (replacement
therapy, e.g., thyroxine, insulin, or physiologic corticosteroid replacement
therapy for adrenal or pituitary insufficiency, is not considered a form of
systemic treatment, and is allowed).
- Evidence of active noninfectious pneumonitis or history of interstitial lung
- A risk of reactivation of hepatitis B or C.
- Previously received an immune therapy that was discontinued due to immune-related
AEs, regardless of grade.
- Uncontrolled endocrine disorder. Patients who are on endocrine replacement
therapy must be on a stable dose.
3. The patient has received treatment with chemotherapy, external-beam radiation, or
other systemic anticancer therapy within 14 days prior to study therapy administration
(42 days for prior nitrosourea or mitomycin-C; patients with advanced prostate cancer
who are receiving luteinizing hormone releasing hormone (LHRH) agonists are permitted
onto the study and should continue use of these agents during study treatment).
4. The patient has received treatment with an investigational systemic anticancer agent
within 14 days prior to study therapy administration.
5. The patient has previously received treatment with RGX-104 or another investigational
agent that is a known LXR agonist.
6. The patient has an additional active malignancy that may confound the assessment of
the study endpoints. Patients with a past cancer history with substantial potential
for recurrence must be discussed with the Medical Monitor before study entry. Patients
with the following concomitant neoplastic diagnoses are eligible: non-melanoma skin
cancer, carcinoma in situ (including transitional cell carcinoma, cervical
intraepithelial neoplasia, and melanoma in situ), organ-confined prostate cancer with
no evidence of progressive disease.
7. The patient has clinically significant cardiovascular disease (e.g., uncontrolled or
any New York Heart Association Class 3 or 4 heart failure (see Appendix 1),
uncontrolled angina, history of myocardial infarction, unstable angina or stroke
within 6 months prior to study entry, uncontrolled hypertension or clinically
significant arrhythmias not controlled by medication).
8. The patient has uncontrolled, clinically significant pulmonary disease (e.g., chronic
obstructive pulmonary disease, pulmonary hypertension) that in the opinion of the
Investigator would put the patient at significant risk for pulmonary complications
during the study.
9. The patient has known active or suspected brain or leptomeningeal metastases. Central
Nervous System (CNS) imaging is not required prior to study entry unless there is a
clinical suspicion of CNS involvement. Patients with stable, treated brain metastases
are eligible provided there is no evidence of CNS disease growth on imaging for at
least 8 weeks following radiation therapy or other locoregional ablative therapy to
10. The patient has a condition requiring systemic treatment with either corticosteroids
(> 10 mg daily prednisone equivalents) or other immunosuppressive medications within
14 days prior to study therapy administration. Inhaled or topical steroids are
permitted in the absence of active autoimmune disease.
11. The patient has uncontrolled intercurrent illness including, but not limited to,
uncontrolled infection requiring therapy, disseminated intravascular coagulation, or
psychiatric illness/social situations that would limit compliance with study
12. The patient is pregnant or breast feeding.
13. The patient has known positive status for human immunodeficiency virus or active or
chronic Hepatitis B or Hepatitis C.
14. The patient is oxygen-dependent.
15. The patient has a history of pancreatitis.
16. The patient has Grade ≥2 hypercholesterolemia (total cholesterol >300 mg/dL or >7.75
mmol/L) and/or hypertriglyceridemia (triglyceride >300 mg/dL or >3.42 mmol/L) in the
17. QTcF >450 msec (males) or >470 msec (females).
18. The patient has a physical abnormality or medical condition that limits swallowing
multiple pills, or has a history of non-adherence to oral therapies.
19. The patient requires statin (e.g., rosuvastatin, atorvastatin, etc.) therapy. If the
patient is taking a statin but discontinuation is considered appropriate, the statin
must be discontinued at least 5 days prior to starting study therapy.
20. The patient requires treatment with a medication that is a strong inhibitor of CYP3A4
(boceprevir, clarithromycin, conivaptan, grapefruit juice, indinavir, itraconazole,
ketoconazole, lopinavir/ritonavir, mibefradil, nefazodone, nelfinavir, posaconazole,
ritonavir, saquinavir, telaprevir, telithromycin, or voriconazole).
21. For the docetaxel escalation stage, patients with NSCLC with alkaline phosphatase >
2.5 × institutional ULN and AST or ALT > 1.5 × institutional ULN.
22. For patients with NSCLC enrolled in the pembrolizumab plus carboplatin/pemetrexed
combination dose escalation or expansion stages:
- The patient received radiation therapy to the lung that is >30 Gray (Gy) within 6
months of the first dose of study medication
- The patient completed palliative radiotherapy ≤7 days of the first dose of study
- The patient has received live-virus vaccination ≤30 days of planned start of
- The patient has clinically active diverticulitis, intra-abdominal abscess,
gastrointestinal obstruction, peritoneal carcinomatosis
- The patient is expected to require any other form of antineoplastic therapy while
- The patient has known hypersensitivity to another monoclonal antibody (mAb)
- The patient has known sensitivity to any component of carboplatin or pemetrexed
- The patient is unable to interrupt aspirin or other nonsteroidal
anti-inflammatory drugs (NSAIDs), other than an aspirin dose ≤1.3 g per day, for
a 5-day period (8-day period for long-acting agents, such as piroxicam)
- The patient is unable or unwilling to take folic acid or vitamin B12
23. The patient has clinical or laboratory evidence of a paraneoplastic syndrome.
24. The patient has experienced weight loss of >10% of their body weight over the
preceding 3 months.
25. The patient has any medical condition which, in the opinion of the Investigator,
places the patient at an unacceptably high risk for toxicities.