Inclusion Criteria:

          1. Patients with retinoblastoma with a somatic mutation of the geneRB1 and active tumor
             in a single eye, or germinal mutation of RB1 with active tumor/s in an eye and the
             contralateral eye unaffected, enucleated or without tumor activity. In both cases,
             relapsed or refractory with the use of systemic, intraarterial or intravitreal
             chemotherapy or radiotherapy, in accordance with the availability at his/her referral
             site, in whom enucleation is the only recommended treatment under opinion of the
             medical team treating the case at the originating referral site.

          2. Normal renal function: serum creatinine: <45μmol/L (0-2 years); <57μmol/L (3-6 years);
             <60μmol/L (7-10 years); <80μmol/L (11-13 years).

          3. Normal Hepatic function: serum ALT: <0,52μkat/L (de 9 months -12 years); serum AST:
             61-80 g/L (8 months - 5 years); 63-83 g/L (5-9 years); 63-82 g/L (9-12 years).

          4. Adequate marrow reserve manifested in an absolute neutrophil count> 1000 / mm3,
             platelets> 100,000 / mm3 and hemoglobin> 8 g / dl, without transfusional or cytokine
             support at least one month prior to study entry.

          5. Age greater than one year and less than 12 years at the time of inclusion in the
             study.

          6. Informed consent form signed.

        Exclusion Criteria:

          1. Presence of factors that require immediate enucleation of the affected eye such as
             glaucoma, rubeosis iridis, anterior chamber involvement.

          2. Comorbidities: Uncontrolled epilepsy with anticonvulsant treatment, cardiac disease
             not compensated by treatment.

          3. Active Infections.

          4. Other chronic or active acute diseases that under the criterion of the researcher were
             an exclusion criterion.

          5. History of having received attenuated or live vaccines in the 30 days prior to
             inclusion in the study.

          6. Any cause of Immunosuppression.

          7. Trilateral Retinoblastoma.

          8. Extraocular spread.

          9. History of having received treatment for retinoblastoma with chemotherapy or radiation
             therapy by any means within 30 days prior to inclusion in the study.

         10. Patients who can not complete the study procedures for reasons psychological or
             social.

         11. Pregnancy. Female patients with procreative potential should be agree to undergo a
             blood or urine pregnancy test and the result should be negative to enter the study.