This is an open-label Phase 1a First-in-Human Study to determine Safety and Tolerability of
ASV® AGEN2017 with QS-21 Stimulon® Adjuvant as a Single Agent in Subjects With Tumors at Risk
of Relapse Undergoing Observation as Standard of Care Following Complete Surgical Resection.
This is a Phase 1a study to evaluate neoantigen vaccine, AutoSynVax (ASV®) AGEN2017 in
subjects with resected solid tumors, no evidence of disease (NED), and with an estimated life
expectancy of ≥12 months from the time tissue has been submitted for vaccine manufacture. A
minimum of 3 subjects will be enrolled to receive every two weeks subcutaneous injection of
AGEN2017 + QS-21 adjuvant.
1. Signed, written informed consent.
2. Age ≥18 years.
3. Diagnosis of solid cancer that has been completely resected, NED, and eligible for
observation only as SOC yet remain at risk of relapse per Investigator discretion.
These include subjects diagnosed with malignant melanoma, non-small cell lung cancer,
bladder cancer, colorectal cancer, breast cancer, renal cancer, head and neck cancer,
cervical cancer, and soft tissue sarcoma.
4. Life expectancy ≥12 months from the time of consent.
5. Available fresh tissue from surgical excision. If fresh tissue is not available,
formalin-fixed paraffin-embedded archival tissue may be used. The modality of the
biopsy (e.g., endobronchial ultrasound, bronchoscopic, computed-tomography-guided
needle biopsy) is not specified; however, core biopsy and fine needle aspiration are
acceptable as long as the biopsy can be prepared as a cell block in paraffin-embedded
6. Eastern Cooperative Oncology Group (ECOG) performance status 0 or 1.
7. Adequate bone marrow function, as measured from studies of peripheral blood (absolute
neutrophil count ≥1,500/mm3, absolute lymphocyte count ≥500/mm3, platelet count
50,000/mm3, hemoglobin >8.0 mg/dL).
8. Adequate cardiac function (New York Heart Association class ≤II).
9. Female subjects of childbearing potential must have a negative serum pregnancy test at
the screening and pretreatment visits, and prior to first dose of study medication.
Non-childbearing potential (other than by medical reasons) is defined as 1 of the
1. ≥45 years of age and amenorrheic for >1 year by self-report.
2. Amenorrheic for >2 years without a hysterectomy and oophorectomy, and
follicle-stimulating hormone value in the postmenopausal range upon screening
3. Status post-hysterectomy, -oophorectomy, or -tubal ligation. If of childbearing
potential, female subjects must be willing to use adequate birth control during
the study, starting with the screening visit through 120 days after the last dose
of study therapy.
Male subjects with a female partner(s) of childbearing potential must agree to use a
condom throughout the trial, starting with the screening visit through 120 days after
the last dose of study therapy. Male subjects with pregnant partners must agree to use
a condom; no additional method of contraception is required for the pregnant partner.
Note: Abstinence is acceptable for both female and male subjects if this is the
subject's established and preferred contraception method.
10. Subject is willing and able to comply with the requirements of the protocol.
1. Subjects must not have received anticancer medications or investigational drugs within
the following intervals before first dose of study drug:
1. ≤14 days for chemotherapy, targeted small molecule therapy, anticancer hormone
therapy, or radiation therapy, with the following exceptions:
- Bisphosphonates and denosumab are permitted.
- Novel imaging agents that have Phase 1 safety data and have not demonstrated
therapeutic activity are permitted.
- Physiologic steroid replacement for adrenal insufficiency (e.g., <10 mg
prednisone per day) is permitted.
- Prophylactic use of inhaled or topical corticosteroid or short course of
intravenous systemic corticosteroid (≤3 days) for radiographic procedures is
- Use of physiologic corticosteroid replacement therapy must be approved by
the medical monitor.
2. ≤28 days for prior cancer immunotherapy.
3. ≤28 days for prior monoclonal antibody used for anticancer therapy, with the
exception of denosumab.
4. ≤7 days for immunosuppressive treatment for any reason. Systemic corticosteroids
are not allowed except as defined above.
5. e. ≤28 days before first dose of study drug for all other investigational study
drugs or devices.
Note: Washout is applicable to the screening visit only, and not applicable prior to
the first dose of study drug. Subjects are not to receive anti-cancer therapies from
date of consent through the post treatment visit (end of treatment or final safety
2. Diagnosis of clinically significant immunodeficiency (as defined by the principal
investigator), or actively receiving or potentially needing any form of
immunosuppressive therapy within 7 days prior to the first dose of study drug until
the end of the trial.
3. History or current evidence of any condition, therapy, or laboratory abnormality that
might confound the results of the trial, interfere with the subject's participation
for the full duration of the trial, or is not in the best interest of the subject to
participate, in the opinion of the treating investigator or medical monitor.
4. Uncontrolled intercurrent illness, including but not limited to uncontrolled
infection, symptomatic congestive heart failure, unstable angina pectoris,
uncontrolled cardiac arrhythmia, or social situations that would limit compliance with
study requirements in the opinion of the treating investigator or medical monitor.
5. History of intolerance or allergic reactions attributed to compounds of similar
chemical or biologic composition to AGEN2017 or QS-21 adjuvant.
6. Women who are pregnant or breastfeeding.