An open label, multi-centre Phase 1/2a study of modified and unmodified autologous Tumour
Infiltrating Lymphocytes (TIL) in patients with platinum-resistant ovarian cancer. The
purpose of this phase I/II study is to evaluate the feasibility and safety of both standard
unmodified TIL (UTIL-01) and TIL engineered to express the co-stimulatory receptor CoStAR
(CoTIL-01) in platinum resistant ovarian cancer.
This is a single-arm Phase 1/2a study of unmodified (UTIL-01) and gene modified (CoTIL-01)
adoptive TIL therapy which will enrol sequentially. A total of 8 patients will be recruited
to the UTIL-01 cohort to receive autologous standard unmodified TIL (phase 2). Up to 14
patients will receive autologous gene engineered TIL(CoTIL-1) in a dose escalation design
(Phase 1/2a). Once patients have met all the pre-screening inclusion criteria, and that
sponsor has confirmed a successful TIL harvest, a request to manufacture will be sent to the
Sponsor to initiate TIL production. Manufacturing and quality control assessment is
anticipated to take approximately 6 weeks. During this time, patients may receive standard of
care chemotherapy (bridging chemotherapy) as deemed appropriate by the treating oncology
team. Patients will proceed to the main trial after completion of bridging chemotherapy. Once
the TIL product is certified for release, and that patient has consented to the main trial
and has completed main trial screening assessments, study treatment can be scheduled.
Patients will receive non-myeloablative lymphodepleting pre-conditioning chemotherapy with
cyclophosphamide 600mg/m2/day and fludarabine 30mg/m2/day on Day -5, -4 and -3. Chemotherapy
will aim to be delivered as an outpatient, but patients can be admitted if clinically needed.
Patients will be required to maintain oral hydration of >2 litres per day. If this is felt to
be difficult to achieve then the patient will be admitted for IV fluids. Patients will be
admitted for TIL infusion on Day 0. The TIL infusion will be administered at least 36 hours
after last dose of chemotherapy. The cells will only be thawed once an Investigator has made
a positive decision to go ahead with infusion and confirmed this in writing. TIL infusion may
be delayed for up to 7 days for clinical reasons or for issues regarding the cell
specification. This decision must be made before final preparation for infusion. Following
TIL infusion, patients will commence subcutaneous interleukin-2 at a fixed dose of 18 million
units once a day. Patients must remain an inpatient for the duration of IL-2 treatment for a
minimum of 7 days post TIL infusion. Recruitment to the study will occur over approximately
24-month period. Recruitment to CoTIL-01 will commence after UTIL-01. Patients will be
followed up in the study for 24 months post TIL infusion.
Inclusion Criteria:
- Criteria for Pre-Screening Phase
Patients are eligible to be included in the pre-screening phase of the study only if all of
the following criteria apply:
1. Women with histologically confirmed recurrent metastatic platinum-resistant high-grade
serous ovarian cancer (HGSOC) (platinum resistant defined as progressing within 6
months of last platinum-containing combination chemotherapy. Patients must have
received at least 1 line of prior platinum-containing combination chemotherapy and
have completed at least 4 cycles of this treatment).
2. Expected to fulfil all entry criteria for OVSTAR Main Study
3. Written informed consent to Pre-Screening
4. Measurable disease by Response Evaluation Criteria in Solid Tumours 1.1
5. Have disease suitable for fresh TIL harvesting from tumour biopsies (only applicable
to patients who are not participants of Sponsor's Tumour Collection Programme, PRIME)
6. Medically suitable for a general anaesthetic and surgical biopsy (only applicable to
patients who are not participants of Sponsor's Tumour Collection Programme, PRIME)
7. Women of child bearing potential must be willing to practise a highly effective method
of birth control once consented to pre-screening
8. World Health Organisation (WHO) Performance Status of 0 or 1 (Appendix 3)
9. Age equal to or greater than 18 years
10. Life expectancy > 6 months
11. Absence of any psychological, familial, sociological or geographical condition
potentially hampering compliance with the Pre-Screening or Main Study protocol and
follow-up schedule; those conditions should be discussed with the patient before
registration in the trial
12. Seronegative for HIV antibody, Hep B antigen, Hep C antibody and syphilis
13. Haematological and biochemical indices
Exclusion Criteria:
- Exclusion for Pre-Screening Phase
Patients will not be invited to participate in Pre-Screening if any of the following
criteria apply:
1. History of a previous malignancy at another site, unless followed for >2 years with no
sign of recurrent disease (local completely excised cutaneous basal cell, squamous
cell carcinoma or in situ carcinoma will be allowed).
2. Patients receiving chemotherapy, targeted therapy, immunotherapy or systemic steroids
including steroid doses >10mg/day of prednisolone (or equivalent) during the previous
four weeks prior to TIL harvesting. Patients who require such therapies intermittently
due to pre-existing disorders are also excluded.
3. Evidence of any active significant infection.
4. Patients who have any malignant or likely malignant Central Nervous System (CNS)
lesion visible on CT.
5. Evidence of clinically significant immunosuppression such as primary immunodeficiency
(e.g. severe combined immunodeficiency disease).
6. Clinically significant cardiac disease. Examples would include unstable coronary
artery disease, myocardial infarction within 6 months or class III or IV American
Heart Association criteria for heart disease.
7. Patients who are at high medical risk because of non-malignant systemic disease
including those with uncontrolled cardiac or respiratory disease, or other serious
medical or psychiatric disorders which, in the lead clinician's opinion, would not
make the patient a good candidate for adoptive TIL therapy.
8. Severe and active autoimmune disease.
9. On concomitant treatment with other experimental drugs within 4 weeks of TIL
harvesting.
10. Patients not considered likely to comply with required follow up.
11. Patients with severe allergies, history of anaphylaxis or known allergies to the
administered drugs.
12. Patients who have received any prior adoptive cell therapy or organ transplant
(including stem cells).
13. Patients who are pregnant or breast feeding should be excluded from pre-screening
14. Patients with any contraindications to any of the components of the study Non
Investigational Medicinal Products (cyclophosphamide, fludarabine, Interleukin-2) will
be excluded
15. Patents who have received live vaccines within 4 weeks prior to TIL therapy will be
excluded
Inclusion for Main Study
-Patients are eligible to be included in the Main Study only if all of the following
criteria, and the inclusion criteria listed in Section 1.3.1, apply:
1. Women with metastatic platinum resistant high-grade serous ovarian cancer (HGSOC) who
have recurrent disease (platinum resistant defined as progressing within 6 months of
last platinum-containing combination chemotherapy. patients must have received at
least 1 line of prior platinum-containing combination chemotherapy and have completed
at least 4 cycles of this treatment).
2. Informed consent to Main Study
3. Confirmation from Sponsor of successful TIL growth
4. Measurable disease (by Response Evaluation Criteria in Solid Tumors 1.1) on CT within
4 weeks of main study entry
5. Left ventricular ejection fraction >50% on Echocardiogram scan
6. Patients must be willing to practice a highly effective method of birth control during
treatment and for four months after receiving the preparative regime if appropriate.
7. World Health Organisation (WHO) Performance Status of 0 or 1 (Appendix 3)
8. Age equal to or greater than 18 years
9. Life expectancy > 6 months
10. Absence of any psychological, familial, sociological or geographical condition
potentially hampering compliance with the Pre-Screening or Main Study protocol and
follow-up schedule; those conditions should be discussed with the patient before
registration in the trial
11. Seronegative for HIV antibody, Hep B antigen, Hep C antibody and syphilis
12. Haematological and biochemical indices
Exclusion for Main Study
1. Patients receiving day 1 of their last cycle of chemotherapy or targeted therapy less
than four weeks prior to pre-conditioning chemotherapy.
2. Patients receiving systemic immunosuppressive therapy including steroids at doses
higher than 10mg/day of prednisolone (or equivalent) within four weeks of commencing
pre-conditioning chemotherapy (unless this is required briefly as anti-emetic
prophylaxis for the treatments detailed in above point 1). Patients who require such
therapies intermittently due to pre-existing disorders are also excluded.
3. Patients who have any malignant or likely malignant Central Nervous System (CNS)
lesion visible on CT.
4. Evidence of any active significant infection.
5. Evidence of clinically significant immunosuppression such as primary immunodeficiency
(e.g. severe combined immunodeficiency disease).
6. Clinically significant cardiac disease. Examples would include unstable coronary
artery disease, myocardial infarction within 6 months or class III or IV American
Heart Association criteria for heart disease.
7. Patients who are at high medical risk because of non-malignant systemic disease
including those with uncontrolled cardiac or respiratory disease, or other serious
medical or psychiatric disorders which, in the lead clinician's opinion, would not
make the patient a good candidate for adoptive TIL therapy.
8. Severe and active autoimmune disease.
9. Receiving concomitant treatment with any other experimental drugs within 4 weeks of
pre-conditioning chemotherapy. Patients receiving experimental immunotherapies will be
discussed with the sponsor.
10. Patients not considered likely to comply with required follow up.
11. Patients with severe allergies, history of anaphylaxis or known allergies to the
administered drugs.
12. Patients who are pregnant or breast feeding should be excluded from entering the study
13. Patients who have received any prior adoptive cell therapy or organ transplant
(including stem cells).
14. Caution should be exercised for patients requiring regular drainage of ascites or
pleural effusions. When there is sufficient fluid to be safely drained, drainage must
be performed prior to trial enrolment and pre-conditioning chemotherapy in those
patients.
15. Patients with any contraindications to any of the components of the study Non
Investigational Medicinal Products (cyclophosphamide, fludarabine, Interleukin-2) will
be excluded
16. Patents who have received live vaccines within 4 weeks prior to TIL therapy will be
excluded.
