Currently, there is no method to predict whether a patient will have benefit from stem cell
transplant or not. The usual approach is to proceed to stem cell transplant as long as a
patient with MM has adequate organ function, meaning heart, kidney, lungs and other organs
can tolerate the complications from transplant.
In this study, all participants will already have had pre-collection bone marrow aspirate as
standard of care that will be available for minimal residual disease (MRD) testing by NGS
platform. Participants will receive only one, low-dose of melphalan (Evomela) intravenously.
Participants will be asked to return to clinic for a follow-up visit at one week and two
weeks after the infusion. Another bone marrow aspirate will be performed after 2 weeks from
the day of Evomela administration. After the study, participants will have a standard-of-care
(SOC) autologous stem cell transplant.
The primary objective is to evaluate the impact of a test dose of low dose melphalan (16
mg/m2) before autologous hematopoietic cell transplant on disease volume measured by Next
Generation Sequencing (NGS).
Secondary objectives are:
- To assess safety of low dose melphalan after CD34 (cluster of differentiation 34)
collection and before high-dose melphalan-based autologous stem cell transplant.
- To describe the impact of a test dose of low dose melphalan (16 mg/m2) before autologous
hematopoietic cell transplant on disease volume measured by peripheral blood Mass
Spectrometry.
Inclusion Criteria:
- Participants must have diagnosis of symptomatic MM
- Participants must have received at least three cycles of anti-myeloma regimen
including a proteasome inhibitor (i.e., bortezomib, carfilzomib or ixazomib) plus
Lenalidomide or daratumumab, and have future plan of autologous stem cell transplant.
- Participants must have achieved Partial Response based on International Myeloma
Working Group response criteria (Appendix II).
- Participants must have at least equal or greater than 100 mg/dL or 0.1 gr/dL
monoclonal protein on serum electrophoresis and immunofixation.
- Minimum 3 x10^6/kg collected CD34+cells in one or multiple days. (CD=cluster of
differentiation)
- Eastern Cooperative Oncology Group (ECOG) performance status 0-2. Life expectancy ≥ 12
months
- Adequate hepatic function, as defined by:
- Serum ALT ≤ 3.5 times the upper limit of normal (ALT=alanine aminotransferase)
- Serum direct bilirubin ≤ 2 mg/dL (34 μmol/L) within 21 days prior to initiation
of therapy
- Creatinine clearance (CrCl) ≥ 40 mL/minute within 21 days prior to start of therapy
either measured or calculated using a standard formula (e.g., Cockcroft and Gault).
- Adequate bone marrow function ,as defined by:
- Hemoglobin ≥ 10.0 g/dl
- WBC (white blood cell count) ≥ 3.00 x 10^9/L
- Absolute neutrophil count ≥1.5 x10^9/L
- Platelets ≥ 100 x 10^9/L ---Growth factors are not allowed to be used in order to
meet adequate bone marrow function
- Written informed consent in accordance with federal, local, and institutional
guidelines
- Participants of childbearing potential must agree to practice reliable contraception
for at least 28 days before and for 60 days after last dose of study drug. Reliable
contraception is defined as:
- One highly effective method and one additional effective (barrier) method:
- Examples of highly effective methods:
- Intrauterine device (IUD)▪Hormonal (injections, implants,
levonorgestrel releasing intrauterine system [IUS], medroxyprogesterone
acetate depot injections, ovulation inhibitory progesterone-only pills
[e.g.desogestrel])
- Tubal ligation
- Partner's vasectomy
- Examples of additional effective methods
- Male condom
- Diaphragm
- Cervical Cap
Exclusion Criteria:
- Prior treatment toxicities have not resolved to ≤ Grade 2 according to NCI CTCAE
Version 5.0 (except neuropathy).
- Participant receiving any other investigational agents within 21 days prior to
study/treatment
- Treatment with any anti-myeloma chemotherapy within 14 days
- Diagnosis of amyloidosis, POEMS.
- Major surgery, radiotherapy or infection requiring therapy within 14 days of starting
study treatment.
- History of allergic reactions attributed to compounds of similar chemical or biologic
composition to melphalan
- Participants with uncontrolled intercurrent illness including, but not limited to
ongoing or active infection, symptomatic congestive heart failure, unstable angina
pectoris, cardiac arrhythmia, or psychiatric illness/social situations that would
limit compliance with study requirements.
- Pregnant or breastfeeding women are excluded from this study because melphalan is an
alkalizing agent with the potential for teratogenic or abortifacient effects. Because
there is unknown, but potential risk for adverse events in nursing infants secondary
to treatment of the mother with melphalan, breastfeeding should be discontinued if the
mother is treated with melphalan
- Unstable angina or myocardial infarction within 4 months prior to registration, NYHA
(New York Heart Association) Class II, III or IV heart failure, uncontrolled angina,
history of severe coronary artery disease, severe uncontrolled ventricular
arrhythmias, sick sinus syndrome, or electrocardiographic evidence of acute ischemia
or Grade 3 conduction system abnormalities unless subject has a pacemaker.
- Cerebrovascular disease manifested as prior stroke at any time or TIA (transient
ischemic attack) in the 12 months prior to initiation of therapy
- Non-hematologic malignancy within the past 3 years with the exception of a) adequately
treated basal cell carcinoma, squamous cell skin cancer, or thyroid cancer;
b)carcinoma in situ of the cervix or breast; c) prostate cancer of Gleason Grade 6 or
less with stable prostate-specific antigen levels or d) cancer considered cured by
surgical resection or unlikely to impact survival during the duration of the study,
such as localized transitional cell carcinoma of the bladder or benign tumors of the
adrenal or pancreas.
- Any other clinically significant medical disease or condition that, in the
Investigator's opinion, may interfere with protocol adherence or a subject's ability
to give informed consent.
